Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L.

Abstract

The objective of this study was to determine the effectiveness and the antioxidant activity of Potentilla recta L. (Rosaceae), which is traditionally used in the treatment of many diseases. against pathogenic microorganisms in the skin. The antioxidant activity was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH center dot) and 2,2-Azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS center dot(+)) radical scavenging activities, inhibition of 6-carotene bleaching, protection of 2-deoxyribose and bovine brain-derived phospholipids against hydroxyl radical-mediated degradation assay. at different concentrations ranging from 0.001 to 2 mg mL(-1). Antimicrobial activity was evaluated against Staphylococcus aureus (ATCC 29213), Staphylococcus eindernnilis (ATCC 3699), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 90028) by microdilution method. Besides. viability enhancing effects on murine fibroblast cells (1.929) were determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The key parameters for the extract included the following: IC50: 0.19 mg mL(-1), TEAC: 0.76 mmol L-1 Trolox, reduction: 0.87 mmol g(-1) AsscE, and protection of lipid peroxidation IC50: 0.07 mg mL(-1). A strong effect on S. epidermidis was observed with 79% inhibition at a concentration of 125 mg mL(-1) but did not show toxicity to L929 cells below 250 mg mL(-1) concentration. The present findings highlight the need for research of plants for traditional medicinal uses and the importance of scientific evaluations.