Fabrication of trichlorovinylsilane-modified-chitosan film with enhanced solubility and antibacterial activity

Abstract

Here, trichlorovinylsilane-modified-chitosan (TVS-m-CS) film is synthesized and explicated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy and X-ray diffraction (XRD). The SEM micrograph of TVS-m-CS revealed smooth structure with bulge surface. The TVS-m-CS antibacterial activity was evaluated using the broth dilution and agar diffusion methods. Results herein indicated that the TVS-m-CS exhibited enhanced water solubility (67 mg/mL) compared to the cross-linked chitosan (CS) film (5.5 mg/mL). Notably, TVS-m-CS showed a significantly enhanced antibacterial activity againstE. coli, S. aureus, E. faecalis, Kleb spp.and methicillin-resistantStaphylococcus aureus(minimum inhibitory concentration [MIC] = 0.078-0.156 mg/mL and minimum bactericidal concentration [MBC] = 12.5-3.12 mg/mL) than the original CS film. Unlike most quaternized chitosan derivatives, the TVS-m-CS was prepared via an easy and simple route and exhibited a comparatively high antibacterial performance. The time-kill study revealed that 5 mg/mL TVS-m-CS exposed to the bacteria cells is sufficient to give a similar to 0.08 log(10) reduction (> 99.9% kill rate) within 24 h.